Toxicity of Dithiothreitol (DTT) to Drosophila melanogaster
نویسندگان
چکیده
منابع مشابه
Evaluation of dithiothreitol (DTT) for inactivation of IgM antibodies.
A newly introduced sulphydryl compound, dithiothreitol (DTT), is evaluated for its optimal conditions of inactivation of IgM antibodies. The maximal effects of DTT reagent are observed when its final concentrations are between 0.0025 M and 0.005 M, pH between 7.0 and 8.0, and incubation at 37 degrees C. Concentrations over 0.01 M, pH values greater than 8.0, and an incubation temperature over 4...
متن کاملEvaluation of dithiothreitol ( DTT ) for inactivation of IgM antibodies 1
A newly introduced sulphydryl compound, dithiothreitol (DTT), is evaluated for its optimal conditions of inactivation of IgM antibodies. The maximal effects of DTT reagent are observed when its final concentrations are between 00025 M and 0005 M, pH between 7 0 and 8O0, and incubation at 370C. Concentrations over 0 01 M, pH values greater than 8-0, and an incubation temperature over 40'C result...
متن کاملMeasuring IgG anti-A/B titres using dithiothreitol (DTT).
In comparing the use of the sulphydryl compounds 2-mercaptoethanol (2ME) and dithiothreitol (DTT) a rapid one-stage technique for titrating IgG anti-A and anti-B using DTT in an antiglobulin test was tried. The results correlated well with those obtained by either of the two-stage methods using 2ME or DTT.
متن کاملUsing Drosophila melanogaster to identify chemotherapy toxicity genes.
The severity of the toxic side effects of chemotherapy shows a great deal of interindividual variability, and much of this variation is likely genetically based. Simple DNA tests predictive of toxic side effects could revolutionize the way chemotherapy is carried out. Due to the challenges in identifying polymorphisms that affect toxicity in humans, we use Drosophila fecundity following oral ex...
متن کاملRedox activity and chemical interactions of metal oxide nano- and micro-particles with dithiothreitol (DTT).
The wide application and production of nanotechnology have increased the interest in studying the toxicity of nano- and micro-sized particles escaping into air from various aspects of the production process. Metal oxides (MOs) are one particular class of particles that exist abundantly in ambient PM. Studies show an emphasis on biological mechanisms by which inhalation exposure to MOs leads to ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Toxicology Reports
سال: 2021
ISSN: 2214-7500
DOI: 10.1016/j.toxrep.2020.12.014